e coli strains cft073  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Comprehensive molecular and phenotypic profiling of uropathogenic Escherichia coli in a Honduran healthcare setting: virulence, resistance and phylogeny

doi: 10.3389/fmicb.2025.1656938

Figure Lengend Snippet: Prevalence of E. coli phylogenetic groups. (A) Prevalence of each phylogenetic group among the total number of clinical isolates analyzed; (B) Prevalence according to the sex of the patient. “?”: Unknown phylogroup.

Article Snippet: Positive controls included DNA from E. coli strains CFT073, ATCC 25922, and UPEC 39, as described in prior publications ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Comprehensive molecular and phenotypic profiling of uropathogenic Escherichia coli in a Honduran healthcare setting: virulence, resistance and phylogeny

doi: 10.3389/fmicb.2025.1656938

Figure Lengend Snippet: Distribution of the total number of antibiotics to which Escherichia coli isolates are resistant, according to their phylogenetic group. (A) All isolates ( n = 126); (B) Isolates from female patients ( n = 99); (C) Isolates from male patients ( n = 27). In each graph, bars indicate the median and interquartile ranges (IQR).

Article Snippet: Positive controls included DNA from E. coli strains CFT073, ATCC 25922, and UPEC 39, as described in prior publications ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Analysis of the prevalence of PAIs (A) and the number of PAIs (B) among commensal Escherichia coli from adults and young children. *Statistically significant.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Analysis of the relative expression level of the siderophore receptor genes fyuA (A) , iutA (B) , iroN (C) , ireA (D) among Escherichia coli isolates from adults and young children. E. coli 85—an isolate of commensal E. coli from our collection, showing expression levels for the remaining genes ( fyuA , iutA , and iroN ) similar to the control UPEC strains—was used as a control for normalization.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques: Expressing, Control

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Distribution of the STs among commensal Escherichia coli isolates from adults (A) and young children (B) .

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: Associations between ST and relative expression of siderophore receptor genes fyuA , iutA , iroN , and ireA among Escherichia coli isolates from adults.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques: Expressing

Journal: Frontiers in Microbiology

Article Title: Extraintestinal traits of pathogenicity and sequence type lineages in commensal Escherichia coli from adults and young children: genotypic and phenotypic profiles

doi: 10.3389/fmicb.2025.1579685

Figure Lengend Snippet: A maximum-likelihood tree depicting the phylogenetic relationships based on the nucleotide sequences of seven housekeeping genes: adk , fumC , gyrB , icd , mdh , purA , and recA among the Escherichia coli isolates from adults (A) and young children (B) . The tree was reconstructed with RAxML, using a general time-reversible nucleotide substitution model (GTRGAMMA) and 1,000 bootstrap replicates. The isolates’ names are indicated at the tip of the tree. Next, the phylogroups with the color displayed in the legend, STs, and clonal complexes are presented. A parts heat map is given on the right side of the figure. The first part, designated as genotype, encompasses PAIs and the VGs clustered according to their function and marked with different colors displayed in the legend. The second part, designated as phenotype, presents phenotypic traits of virulence, marked with colors corresponding to the genotype in the legend. The degree of biofilm formation was classified into three categories: S, strong; M, moderate; and W, weak.

Article Snippet: Uropathogenic E. coli strain CFT073 American Type Culture Collection (ATCC) 700,928 (Argenta, Poznań, Poland), E. coli strains from the ECOR collection (Institut Pasteur Collection, Paris, France) and from our collection of human fecal strains, known to possess (validated by sequencing) or lack the genes of interest, were examined in all PCR reactions as positive and negative controls, respectively.

Techniques: